Lapin anti-Humain IgG (F(ab')2 Region) Anticorps

N° du produit ABIN101618

N° du produit (Fournisseur): 609-4104

Aperçu rapide pour Lapin anti-Humain IgG (F(ab')2 Region) Anticorps (ABIN101618)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: F(ab')2 Region

Reactivité: Humain

Hôte: Lapin

N° du produit (Fournisseur): 609-4104

Fournisseur: Rockland

Fonction: Human IgG F(ab')2 Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c).

Attributs du produit: Anti-Human IgG F(c) generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Human IgG F(ab')2 fragment

Information d'application

Indications d'application: Application Note: Anti-Human IgG F(ab')2 antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:25,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 6.03 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.