Lapin anti-Humain IgG (F(ab')2 Region) Anticorps (HRP) - Preadsorbed

N° du produit ABIN101631

N° du produit (Fournisseur): 609-4318

Aperçu rapide pour Lapin anti-Humain IgG (F(ab')2 Region) Anticorps (HRP) - Preadsorbed (ABIN101631)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: F(ab')2 Region

Reactivité: Humain

Hôte: Lapin

N° du produit (Fournisseur): 609-4318

Fournisseur: Rockland

Fonction: Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Attributs du produit: Anti-Human IgG (H&L) Peroxidase generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Human IgG F(ab')2 fragment

Information d'application

Indications d'application: Application Note: Anti-Human IgG F(ab')2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 μg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product. This product is also suitable for Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Human IgG F(ab')2 Peroxidase Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.