Lapin anti-Humain IgG (Fc Region) Anticorps (HRP)

N° du produit ABIN101672

N° du produit (Fournisseur): 609-4303

Aperçu rapide pour Lapin anti-Humain IgG (Fc Region) Anticorps (HRP) (ABIN101672)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Lapin

N° du produit (Fournisseur): 609-4303

Fournisseur: Rockland

Fonction: Human IgG Fc Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab).

Attributs du produit: Anti-Human IgG (H&L) Peroxidase generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.

Immunogène: Optional[Immunogen]: Human IgG F(c) fragment

Information d'application

Indications d'application: Application Note: Anti-Human IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Abdiche, Harriman, Deng, Yeung, Miles, Morishige, Boustany, Zhu, Izquierdo, Harriman: "Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms." dans: mAbs, Vol. 8, Issue 2, pp. 264-77, (2016) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.