Suitable for Immunohistochemistry on Frozen (1 μg/mL, 1/400) and Paraffin EmbeddedSections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Protocole
Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature
1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1 h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL distilled water.
Concentration
0.4 mg/mL
Buffer
PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
Biohazard Informations: This product contains thimerosal which is hazardous.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use