Application: suitable for highly specific immunological methods requiring extremely lowbackground levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer andspecificity. Recommended Dilution: ELISA: 1:25,000 Western Blot: 1:1,000 - 1:5,000Immunohistochemistry: 1:500 - 1:2,500
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Avoid repeated thawing and freezing.
Stock
4 °C/-20 °C
Stockage commentaire
Store original vial at 2-8 °C and stock solution or aliquots thereof at -28 °C.