Chèvre anti-Humain IgG (Fc Region) Anticorps - Preadsorbed

N° du produit ABIN129737

N° du produit (Fournisseur): 609-101-017

Aperçu rapide pour Chèvre anti-Humain IgG (Fc Region) Anticorps - Preadsorbed (ABIN129737)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 609-101-017

Fournisseur: Rockland

Fonction: Human IgG Fc Antibody Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins. Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.

Attributs du produit: The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to rhodamine.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Human IgG F(c) fragment

Information d'application

Indications d'application: Application Note: Anti-Human IgG F(c) antibody has been tested by dot blot and ELISA. This antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:15,000 - 1:60,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.