Souris anti-Humain IgG (Fc Region) Anticorps

N° du produit ABIN7477947

Aperçu rapide pour Souris anti-Humain IgG (Fc Region) Anticorps (ABIN7477947)

Antigène: IgG

Clonalité: Monoclonal

Application: ELISA

Clone: 27B11E9

Isotype: IgG1 kappa

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Specificité: Human IgG (Fc) Antibody, mAb, Mouse recognizes human IgG and human Fc-tagged proteins.

Attributs du produit: Human IgG (Fc) Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human Fc-tagged protein.

Purification: Protein A affinity column

Immunogène: Human Fc-tagged protein

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0.1 µg/mL
Western Blot: 1 µg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Human IgG (Fc) Antibody, mAb, Mouse is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Human IgG is the major immunoglobulin in blood and plays a key role in the humoral immune response. The Fc portion of human IgG binds to Fc receptor that can activate the complement system. Fc-tagged proteins are generated with DNA recombinant technology. The protein of interest is fused with the Fc tag. Most vectors encoding for Fc tag sequence use the Fc region of human IgG1. Protein A can be used for the purification of Fc-tagged proteins. Human IgG (Fc), mAb, Mouse is a useful tool for immunoassay of human Fc-tagged proteins and human IgG