Chèvre anti-Humain IgG Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN336567

Aperçu rapide pour Chèvre anti-Humain IgG Anticorps (Alkaline Phosphatase (AP)) (ABIN336567)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Détail du produit

Reactivité: Humain

Hôte: Chèvre

Fonction: Goat anti-Human IgG (H&L) - Affinity Pure, ALP Conjugate

Specificité: Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG • light chains on all human immunoglobulins

Réactivité croisée (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins

Purification: Affinity purified using solid phase Human IgG

Pureté: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogène: Purified Human IgG, whole molecule

Information d'application

Commentaires:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody