Aperçu rapide pour Chèvre anti-Humain IgG Anticorps (Alkaline Phosphatase (AP)) (ABIN336609)
Antigène
IgG
Fragment
Fc fragment
Clonalité
Polyclonal
Conjugué
Alkaline Phosphatase (AP)
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)
Reactivité
Humain
Hôte
Chèvre
Fonction
Goat anti-Human IgG Fc - Affinity Pure, ALP Conjugate, min x w/human IgA + IgM
Specificité
Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG
Réactivité croisée (Details)
Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgA or IgM
Purification
Affinity purified using solid phase Human IgG
Pureté
Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry
Restrictions
For Research Use only
Format
Liquid
Concentration
1.5 mg/mL
Buffer
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Stock
4 °C,-20 °C
Stockage commentaire
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.