Chèvre anti-Humain IgG (Fab Region) Anticorps (HRP) - Preadsorbed

N° du produit ABIN457991

Aperçu rapide pour Chèvre anti-Humain IgG (Fab Region) Anticorps (HRP) - Preadsorbed (ABIN457991)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Détail du produit

Épitope: Fab Region

Reactivité: Humain

Hôte: Chèvre

Fonction: Goat anti Human IgG Fab, conjugated with Horseradish peroxidase

Specificité: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human Fab of IgG.

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled human serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Igbound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotypespecific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogène: Purified normal Fab of polyclonal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with he antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody