Souris anti-Humain IgG Anticorps (CF®594)

N° du produit ABIN6154807

Détail du produit

Antigène: IgG

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®594

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Mouse Monoclonal anti-Human IgG Immunoglobulin (B33/20), CF594 Conjugate

Attributs du produit: This antibody recognizes a protein of 75 kDa, identified as γ heavy chain of human immunoglobulins. Its epitope maps in CH2 domain of Fc region of IgG. It reacts with all sub-classes of γ chain of human immunoglobulins. It does not cross-react with α (IgA), μ (IgM), ε (IgE), or δ (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin?s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified polyclonal human Ig Gamma Chain

Clone: B33-20

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin/paraffin 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détail du antigène

Antigène: IgG

Autre désignation: IgG Immunoglobulin (IgG Produits)

Classe de substances: Antibody

Poids moléculaire: 75 kDa

ID gène: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861