Anti-Human IgE Secondary Antibodies

Choose from a handpicked selection of anti-human secondary antibodies with enzymatic conjugates like AP, Biotin or HRP or fluorescent conjugates including FITC, APC and DyLight and Atto dye families. Browse our offer for a variety of different host, isotypes or binding specifities.

Unconjugated Anti-Human IgE Secondary Antibodies

Anti-Human IgE Secondary Antibodies with Enzymatic Conjugates

Anti-Human IgE Secondary Antibodies with Fluorescent Conjugates

Secondary antibodies conjugated to fluorescent proteins, eg FITC, TRITC, APC or PE or commercial dye families excel in flow cytometry or immunofluorescence experiments. For example the FITC conjugated Goat Anti-Rabbit IgE. DyLight flurorescent conjugates are an excellent alternative to IRDye550CW or IRDye680CW, they offer comparable excitation and emission spectra and are claimed to be more photostable.

IgE Role in Allergies

IgE that can specifically recognise an allergen (typically this is a protein, such as dust mite or ragweed pollen) has a unique interaction with its high-affinity receptor FcεRI lasting for years. IgE can upregulate the expression of both types of Fcε receptors. Basals and mast cells, capable of mediating inflammatory reactions, become "primed", ready to release messengers like histamine, leukotrienes, and certain interleukins which both help the mast cells survive and accumulate in tissue.

Atopic individuals can have up to ten times the normal level of IgE in their blood; asthmatics however show only local IgE production in the nasal mucosa. Symptoms associated with allergy are airway constriction in asthma, local inflammation in eczema, increased mucus secretion in allergic rhinitis, and increased vascular permeability, presumably to allow other immune cells to gain access to tissues, but which can lead to a potentially fatal drop in blood pressure as in anaphylaxis.

IgE in Autoimmune Disorders

IgE is known to be elevated in various autoimmune disorders such as T-cell defect, hyper IgE syndrome, SLE, rheumatoid arthritis, and psoriasis. Regulation of IgE levels through control of B cell differentiation to antibody-secreting plasma cells is thought to involve the "low-affinity" receptor FcεRII, or CD23. CD23 may also allow facilitated antigen presentation, an IgE-dependent mechanism whereby B cells expressing CD23 are able to present allergen to (and stimulate) specific T helper cells, causing the perpetuation of a Th2 response, one of the hallmarks of which is the production of more antibodies.

IgE in Medical Diagnostics

The concentration of total IgE plays a significant role in the evaluation of allergic diseases, especially eosinophilic pulmonary infiltrates, eosinophilic gastroenteritis and allergic alveolitis like farmer's lung, vasculitides such as granulomatosis with polyangiitis, Churg-Strauss syndrome, and allergic bronchopulmonary aspergillosis. IgE is also often elevated in parasitoses. IgE determination is useful in immunodeficiencies such as T-cell defect, hyper IgE syndrome or rheumatoid arthritis, but also in forms of urticaria, atopic dermatitis, in Quincke's edema, or in unclear exanthema.

Human IgE Secondaries