NLRP3 anticorps (AA 82-93)
Aperçu rapide pour NLRP3 anticorps (AA 82-93) (ABIN297366)
Antigène
Voir toutes NLRP3 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 82-93
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Specificité
- Human NLRP3. This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
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Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bat, Panda, Pig (92%) Dog, Bovine, Rabbit (83%).
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Purification
- Immunoaffinity purified
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Immunogène
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Synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bat, Panda, Pig (92%), Dog, Bovine, Rabbit (83%).
Type of Immunogen: Synthetic peptide
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Indications d'application
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store at -20°C. Avoid freeze-thaw cycles.
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- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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Autre désignation
- NALP3 / NLRP3
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Sujet
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Name/Gene ID: NLRP3
Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS -
ID gène
- 114548
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NCBI Accession
- NP_004886, NP_899632, NP_001230062, NP_001120933, NP_001120934
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UniProt
- Q96P20
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
Antigène
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