NEDD9 anticorps (AA 82-398)
(1 reference)
Aperçu rapide pour NEDD9 anticorps (AA 82-398) (ABIN129519)
Antigène
Voir toutes NEDD9 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 82-398
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N° du produit (Fournisseur)
- 200-301-912
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Fournisseur
- Rockland
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Fonction
- HEF1 Antibody
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Réactivité croisée (Details)
- This Protein A purified antibody is directed against human HEF1 protein.
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Attributs du produit
- Synonyms: mouse anti-hEF1 antibody, mouse anti-NEDD-9 antibody, mouse anti-CASL antibody, Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody
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Purification
- The product was purified from tissue culture supernatant by chromatography.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein.
Immunogen Type: Conjugated Peptide
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Isotype
- IgG2b kappa
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 60-174) antibody
NEDD9 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (Internal Region) antibodyNEDD9 Reactivité: Humain, Rat, Souris WB, ELISA, IHC Hôte: Lapin Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 101-200) antibodyNEDD9 Reactivité: Humain WB, ELISA, IF Hôte: Souris Monoclonal 1B4 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) antibodyKD Validated NEDD9 Reactivité: Humain WB, FACS, IF (p) Hôte: Souris Monoclonal 25GB670 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 82-398) antibodyNEDD9 Reactivité: Humain, Rat, Souris WB, IF, IP, FACS, ICC, IHC (fro) Hôte: Souris Monoclonal 2G9 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 1-834) antibodyNEDD9 Reactivité: Humain WB, IP Hôte: Lapin Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 1-834) antibodyNEDD9 Reactivité: Humain WB, IF Hôte: Souris Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 82-398) antibodyNEDD9 Reactivité: Humain, Rat, Souris WB, ELISA, IF, IP, FM Hôte: Souris Monoclonal 2G9 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) antibodyNEDD9 Reactivité: Humain, Rat IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 150-200) antibodyNEDD9 Reactivité: Humain, Souris WB, IP Hôte: Lapin Monoclonal BLR248L unconjugated Recombinant Antibody
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Indications d'application
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Application Note: This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence. This clone recognizes HEF1 under non-denaturing conditions. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract. This antibody does not recognize p130Cas. Sin1 has not been tested. IF was performed using 4 % PFA fixed cells. This monoclonal mostly detects HEF1 localized at the focal adhesion sites.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:100
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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: "The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome." dans: Nature cell biology, Vol. 7, Issue 10, pp. 937-46, (2005) (PubMed).
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- NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
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Autre désignation
- NEDD9
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Sujet
- Background: HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene. HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1. This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue. HEF1 is also detected in T-cells, B-cells and diverse cell lines. HEF1 is activated upon induction of cell growth. Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detects. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associate with the mitosis spindle.
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ID gène
- 4739, 5453680
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UniProt
- Q14511
Antigène
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