ATP6AP1 anticorps
(8 références)Aperçu rapide pour ATP6AP1 anticorps (ABIN249357)
Antigène
Voir toutes ATP6AP1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Réactivité croisée
- Soja (Glycine max)
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Réactivité croisée (Details)
- No cross-reactivity with: Aspergillus niger
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Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 95 kDa (Arabidopsis thaliana)
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Purification
- serum
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Immunogène
- KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
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anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (AA 51-150) antibody
ATP6AP1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 3A2 unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (AA 90-390) antibodyATP6AP1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (AA 1-470) antibodyATP6AP1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (AA 51-150) antibodyATP6AP1 Reactivité: Humain WB, ELISA Hôte: Souris Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (Internal Region) antibodyATP6AP1 Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (AA 251-300) antibodyATP6AP1 Reactivité: Humain, Souris, Rat, Chien, Cheval, Lapin, Cobaye, Hamster, Roussette (Chauve-souris) WB Hôte: Lapin Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) antibodyATP6AP1 Reactivité: Humain WB, ELISA Hôte: Lapin Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1) (Middle Region) antibodyATP6AP1 Reactivité: Humain, Souris, Rat, Chien, Cheval, Lapin, Cobaye WB Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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Recommended Dilution: 1 : 1000 - 1 : 5000 with standard ECL OR 1 : 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1 : 600 - 1 : 1000 (IF).
Cellular [compartment marker] for plasma membrane -
Commentaires
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for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water.
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Buffer
- PBS pH 7.4
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Conseil sur la manipulation
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Stock
- -20 °C
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Stockage commentaire
- Do not store this antibody at 4 °C.
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: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." dans: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
: "Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family." dans: Journal of experimental botany, Vol. 64, Issue 14, pp. 4375-87, (2013) (PubMed).
: "Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein." dans: Journal of proteomics, Vol. 80C, pp. 66-77, (2013) (PubMed).
: "Plasma membrane H(+) -ATPase gene expression, protein level and activity in growing and non-growing regions of barley (Hordeum vulgare) leaves." dans: Physiologia plantarum, Vol. 144, Issue 4, pp. 382-93, (2012) (PubMed).
: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." dans: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." dans: Journal of proteome research, (2010) (PubMed).
: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." dans: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).
: "Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation." dans: The Plant cell, Vol. 21, Issue 3, pp. 928-43, (2009) (PubMed).
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- ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))
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Autre désignation
- H+ATPase
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Sujet
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AGI Code: At2g18960
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves. -
Poids moléculaire
- 95 kDa (Arabidopsis thaliana)
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UniProt
- P20649
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Pathways
- Proton Transport, SARS-CoV-2 Protein Interactome
Antigène
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