CDKN1B anticorps
Aperçu rapide pour CDKN1B anticorps (ABIN498982)
Antigène
Voir toutes CDKN1B AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of p27 protein. (region surrounding Ala181)
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- Affinity chromatography
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Pureté
- > 95 % by SDS-PAGE
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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B Reactivité: Humain WB, ELISA, IF, IHC (p), PLA Hôte: Souris Monoclonal 4B4-E6 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 150-198) antibodyCDKN1B Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyKO Validated CDKN1B Reactivité: Humain WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B Reactivité: Humain, Souris, Rat, Singe WB, IHC, IF, FACS Hôte: Souris Monoclonal SX53G8 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyKD Validated CDKN1B Reactivité: Humain WB, FACS, ICC Hôte: Lapin Monoclonal 23GB1825 unconjugated Recombinant Antibody
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B Reactivité: Humain, Souris, Rat WB, IF, FACS, IHC (p) Hôte: Souris Monoclonal DCS-72-F6-KIP1-769 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibodyCDKN1B Reactivité: Humain WB, IF Hôte: Souris Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibodyCDKN1B Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B Reactivité: Humain, Souris, Rat, Singe WB, IHC, IF, FACS Hôte: Souris Monoclonal DCS-72-F6 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B Reactivité: Humain WB, IHC, ELISA, IF Hôte: Lapin Monoclonal 1G2 unconjugated Recombinant Antibody
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Indications d'application
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1,0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Autre désignation
- CDKN1B / KIP1
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Sujet
- Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4.p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
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Poids moléculaire
- approx. 27 kDa
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ID gène
- 1027
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NCBI Accession
- NP_004055
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UniProt
- P46527
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Pathways
- Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
Antigène
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