Cullin 1 anticorps
Aperçu rapide pour Cullin 1 anticorps (ABIN265375)
Antigène
Voir toutes Cullin 1 (CUL1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of Cullin-1 protein (region surrounding Tyr761).
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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Pureté
- > 95 % pure by SDS-PAGE
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anti-Cullin 1 (CUL1) (AA 500-776) antibody
CUL1 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyKD Validated CUL1 Reactivité: Humain WB, ICC, FACS Hôte: Lapin Monoclonal 23GB1555 unconjugated Recombinant Antibody
anti-Cullin 1 (CUL1) antibodyCUL1 Reactivité: Humain, Souris WB, IHC, IF, ELISA, ICC Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 Reactivité: Humain WB, IHC, IF, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-250) antibodyCUL1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-776) antibodyCUL1 Reactivité: Humain WB, IF, PLA Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 Reactivité: Humain, Souris, Rat WB, IHC, IF, ELISA, ICC Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivité: Humain, Souris WB, IHC, IF, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivité: Humain WB, IHC, ELISA, IP, IHC (p) Hôte: Souris Monoclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivité: Humain WB, IHC, IF, ELISA Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Cullin 1 (CUL1)
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Autre désignation
- Cullin 1
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Sujet
- CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.Synonyms: CUL-1, CUL1, Cullin-1
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Poids moléculaire
- approx. 90 kDa
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ID gène
- 8454
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NCBI Accession
- NP_003583
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UniProt
- Q13616
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Pathways
- Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases, Regulation of Cell Size
Antigène
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