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SYVN1 anticorps (C-Term)

Cité dans 12 publications. Cet anticorps anti-SYVN1 Polyclonal Lapin (Clone RB4822) (ABIN388980) détecte spécifiquement SYVN1 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) antibody. (SYVN1 anticorps (C-Term))

Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. (SYVN1 anticorps (C-Term))

All lanes : Anti-HRD1 Antibody at 1:2000 dilution Lane 1: D whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse spleen lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. (SYVN1 anticorps (C-Term))

Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term) (ABIN388980 and ABIN2837882) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SYVN1 anticorps (C-Term))

IHC (p)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SYVN1 anticorps (C-Term))

IHC (p)

6 images

(12 références)

N° du produit ABIN388980

434,77 €

Plus frais de livraison 40,00 € et TVA

Destination: France

Envoi sous 14 à 17 jours ouvrables

Aperçu rapide pour SYVN1 anticorps (C-Term) (ABIN388980)

Antigène

Voir toutes SYVN1 Anticorps

SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivité

Humain, Souris

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp SYVN1 est non-conjugé

Application

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB4822

Épitope
16

7

5

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 586-617, C-Term

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1).

Isotype

Ig Fraction
anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 586-617) antibody
SYVN1 Reactivité: Humain, Souris WB, ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
Verified SYVN1 Reactivité: Humain, Rat WB, ELISA, FACS, IF Hôte: Chèvre Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 531-617) antibody
SYVN1 Reactivité: Humain, Souris WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
SYVN1 Reactivité: Humain, Souris, Rat WB, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 58-88), (N-Term) antibody
SYVN1 Reactivité: Humain WB Hôte: Lapin Polyclonal RB5086 unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 1-617) antibody
SYVN1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 238-318) antibody
SYVN1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 4H4 unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 338-487) antibody
SYVN1 Reactivité: Humain ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
SYVN1 Reactivité: Humain, Souris, Rat, Cheval, Chien, Boeuf (Vache), Lapin, Roussette (Chauve-souris), Singe, Hamster, Xenopus laevis, Poisson zèbre (Danio rerio) IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 68-604) antibody
SYVN1 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IF, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

IF: 1:200. WB: 1:2000. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Avoid freeze-thaw cycles.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Date de péremption

6 months
Peters, Déry, LeBlanc: "Familial prion protein mutants inhibit Hrd1-mediated retrotranslocation of misfolded proteins by depleting misfolded protein sensor BiP." dans: Human molecular genetics, Vol. 25, Issue 5, pp. 976-88, (2016) (PubMed).

Jung, Hong, Kim, Mook-Jung: "Acute ER stress regulates amyloid precursor protein processing through ubiquitin-dependent degradation." dans: Scientific reports, Vol. 5, pp. 8805, (2015) (PubMed).

Grotzke, Lu, Cresswell: "Deglycosylation-dependent fluorescent proteins provide unique tools for the study of ER-associated degradation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 9, pp. 3393-8, (2013) (PubMed).

Gharanei, Zatyka, Astuti, Fenton, Sik, Nagy, Barrett: "Vacuolar-type H+-ATPase V1A subunit is a molecular partner of Wolfram syndrome 1 (WFS1) protein, which regulates its expression and stability." dans: Human molecular genetics, Vol. 22, Issue 2, pp. 203-17, (2012) (PubMed).

Hibino, Sugiura, Muro, Shimoyama, Tomita: "Cyclosporin A induces the unfolded protein response in keratinocytes." dans: Archives of dermatological research, Vol. 303, Issue 7, pp. 481-9, (2011) (PubMed).

Lee, Hammerle, Andrews, Stokes, Mustelin, Silva, Black, Doedens: "Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels." dans: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41530-8, (2011) (PubMed).

Kaneko, Koike, Saito, Kitamura, Okuma, Nomura: "Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 11, pp. 3924-32, (2010) (PubMed).

Saito, Kaneko, Okuma, Nomura: "Correlation between decrease in protein levels of ubiquitin ligase HRD1 and amyloid-beta production." dans: Journal of pharmacological sciences, Vol. 113, Issue 3, pp. 285-8, (2010) (PubMed).

Sugiura, Muro, Futamura, Matsumoto, Hashimoto, Nishizawa, Nagasaka, Saito, Tomita, Usukura: "The unfolded protein response is activated in differentiating epidermal keratinocytes." dans: The Journal of investigative dermatology, Vol. 129, Issue 9, pp. 2126-35, (2009) (PubMed).

Maeda, Marutani, Zou, Araki, Tanabe, Yagishita, Yamano, Amano, Michikawa, Nakajima, Komano: "An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid beta-protein." dans: The FEBS journal, Vol. 276, Issue 20, pp. 5832-40, (2009) (PubMed).

Toh, Marotte, Blond, Jhumka, Eljaafari, Mougin, Miossec: "Overexpression of synoviolin in peripheral blood and synoviocytes from rheumatoid arthritis patients and continued elevation in nonresponders to infliximab treatment." dans: Arthritis and rheumatism, Vol. 54, Issue 7, pp. 2109-18, (2006) (PubMed).

Yamada, Ishihara, Tamura, Takahashi, Yamaguchi, Takei, Tokita, Satake, Tashiro, Katagiri, Aburatani, Miyazaki, Oka: "WFS1-deficiency increases endoplasmic reticulum stress, impairs cell cycle progression and triggers the apoptotic pathway specifically in pancreatic beta-cells." dans: Human molecular genetics, Vol. 15, Issue 10, pp. 1600-9, (2006) (PubMed).
Antigène

SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Autre désignation

SYVN1 (HRD1)

Sujet

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.

Poids moléculaire

67685

ID gène

84447

NCBI Accession

NP_115807, NP_757385

UniProt

Q86TM6

Pathways

ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling