TRKB anticorps (AA 1-429)
Aperçu rapide pour TRKB anticorps (AA 1-429) (ABIN337266)
Antigène
Voir toutes TRKB (NTRK2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 1-429
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Specificité
- Recognizes rat TrkB, Mr 145kD, as well as an additional protein, Mr ~90kD, which is likely a TrkB degradation product. Species sequence homology: mouse 97 % and human 88 %.
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Purification
- Protein A purified
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Immunogène
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The entire extracellular domain (corresponding to residues 1-429) of the rat TrkB receptor, expressed in COS cells.
Type of Immunogen: Cells -
Isotype
- IgG
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Indications d'application
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Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 0.5-2 μg/mL detected TrkB in mouse brain membrane protein preparations. -
Commentaires
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
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Autre désignation
- NTRK2 / TRKB
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Sujet
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Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase
Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B -
ID gène
- 4915
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UniProt
- Q16620
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Pathways
- Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
Antigène
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