p130 anticorps (C-Term)

N° du produit ABIN116943

Détail du produit anti-p130 anticorps

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Épitope: C-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p130 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Antiserum will specifically react with a 130 kDa Rb2 protein from Human, Rat and Mouse tissue. No reaction was observed against other related tumor supressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur.

Purification: Delipidation and Defibrination.

Immunogène: Rb2 (p130) peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Genename: RBL2

Information d'application

Indications d'application: Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunologicalmethods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/500-1/20,000. Western Blot: 1/500-1/2,000. Immunoprecipitation: 1/100. Immunohistochemistry on Paraffin Embedded Tissue: 1/200-1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur p130

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Autre désignation: RBL2 / p130 (RBL2 Produits)

Synonymes: anticorps RBL2, anticorps RBR2, anticorps RRB2a, anticorps PRB2, anticorps RBR-2, anticorps Rb2, anticorps p130, anticorps P130, anticorps retinoblastoma-like 2 (p130), anticorps RB transcriptional corepressor like 2, anticorps related to retinoblastoma 2, anticorps RBL2, anticorps LOC541652, anticorps Rbl2

Sujet: P130/pRb2, p107 and Rb together make up the Rb family of cell cycle regulatory proteins. As a family of proteins, the different members share several structural and functional features which include two distinct "pocket domains", interaction with viral gene products, interaction with members of the E2F family of proteins and changes in phosphorylation states leading to modulation of activity and cell cycle expression. The p130 protein is ubiquitously expressed with highest levels in terminally differentiated cells where it presumably functions to maintain the cells in a G0 state and as such has been used as a marker for G0. Depending on position within the cell cycle, p130 can exist in 4 different forms as a function of phosphorylation with Form 0 being the unphosphorylated form and the other three forms being referred to as Forms 1-3 correlating with increasing degrees of phosphorylation. Changes in levels of p130 expression have been reported in several cancers including endometrial, small cell lung, and neuroblastoma.Synonyms: 130 kDa retinoblastoma-associated protein, RB2, RBR-2, Retinoblastoma-like protein 2, Retinoblastoma-related protein 2, p130, pRb2

ID gène: 5934

NCBI Accession: NP_005602

UniProt: Q08999

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases