His Tag anticorps (HRP)
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- Antigène Voir toutes His Tag Anticorps
- His Tag
- Reactivité
- Veuillez nous consulter SVP
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp His Tag est conjugé à/à la HRP
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Séquence
- HHHHHH
- Purification
- Affinity Chromatography on Protein G
- Immunogène
- PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Clone
- AD1-1-10
- Isotype
- IgG1
- Top Product
- Discover our top product His Tag Anticorps primaire
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- Indications d'application
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ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- Antigène
- His Tag
- Autre désignation
- 6xHistidine Epitope Tag (His Tag Produits)
- Classe de substances
- Tag
- Sujet
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
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