GRP94 anticorps (Internal Region)

N° du produit ABIN185655

Cet anticorps anti-GRP94 Polyclonal Chèvre (ABIN185655) détecte spécifiquement GRP94 dans WB, IHC, FACS, IF et ELISA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour GRP94 anticorps (Internal Region) (ABIN185655)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-GRP94 anticorps

Épitope: Internal Region

Fonction: GPR94 / TRA1

Séquence: KEGVKFDESE KTKE

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat, Poisson zèbre (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5 kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecula

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: HSP90B1

Sujet: TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1

ID gène: 7184, 22027, 362862

NCBI Accession: NP_003290

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades