The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested by ELISA detection. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Human IL-4 - Biotin - Clone MP4-25D2 - Isotype Rat IgG1 - Reactivity Hu - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Jung, Schauer, Rieger, Wagner, Einsle, Neumann, Heusser: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." dans: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, (1995) (PubMed).
Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." dans: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, (1993) (PubMed).
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." dans: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, (1989) (PubMed).