MIF anticorps (AA 103-115)

N° du produit ABIN337308

Cet anticorps anti-MIF Polyclonal Chèvre (ABIN337308) détecte spécifiquement MIF dans WB, IHC, ELISA et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Singe.

Aperçu rapide pour MIF anticorps (AA 103-115) (ABIN337308)

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MIF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MIF anticorps

Épitope: AA 103-115

Specificité: Human MIF.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%) Hamster (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-NAANVGWNNSTFA from the C-terminus of human MIF (NP_002406.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MIF

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Autre désignation: MIF

Sujet: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

ID gène: 4282

NCBI Accession: NP_002406

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling