MIF anticorps (AA 2-115)
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- Antigène Voir toutes MIF Anticorps
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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Épitope
- AA 2-115
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp MIF est non-conjugé
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB, IHC-P, ICC in Human.
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
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Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Protein Name: Macrophage migration inhibitory factor - Purification
- Immunogen affinity purified.
- Immunogène
- E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.
- Isotype
- IgG
- Top Product
- Discover our top product MIF Anticorps primaire
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- Indications d'application
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MIF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Antigène
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- Autre désignation
- MIF (MIF Produits)
- Synonymes
- anticorps mif, anticorps Mif, anticorps gif, anticorps glif, anticorps mmif, anticorps LOC100136498, anticorps LOC100284350, anticorps LOC100284546, anticorps GIF, anticorps Glif, anticorps GLIF, anticorps MMIF, anticorps macrophage migration inhibitory factor L homeolog, anticorps macrophage migration inhibitory factor, anticorps macrophage migration inhibitory factor (glycosylation-inhibiting factor), anticorps mif.L, anticorps mif, anticorps MIF, anticorps Mif, anticorps PHATRDRAFT_49660, anticorps LOC100136498, anticorps cl405_1, anticorps LOC100284546
- Sujet
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Macrophage migration inhibitory factor (MIF or MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. The localization of the human gene for MIF is to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.
Synonyms: acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody|GIF antibody|GLIF antibody|Glycosylation inhibiting factor antibody|Glycosylation-inhibiting factor antibody|L-dopachrome isomerase antibody|L-dopachrome tautomerase antibody|Macrophage migration inhibitory factor antibody|Macrophage migration inhibitory factor antibody|MIF antibody|MIF antibody|MIF protein antibody|MIF_HUMAN antibody|MMIF antibody|Phenylpyruvate tautomerase antibody|Phenylpyruvate tautomerase antibody - ID gène
- 4282
- UniProt
- P14174
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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