Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
Specificité
ATR antibody was raised against a peptide corresponding to 13 amino acids near the C-terminus of human ATR. ATR will recognize only the largest isoform.
ANTXR1
Reactivité: Humain
DB
Hôte: Lapin
Polyclonal
RB15324
unconjugated
Indications d'application
ELISA. Western Blot: ATR antibody can be used for detection of ATR by Western blot at 1 to 2 μg/mL. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8) (1). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin (2). Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF) (3). This complex is then endocytosed by the cell, acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis (4).Synonyms: ANTXR1, ATR, TEM8, Tumor endothelial marker 8