BCL6 anticorps

N° du produit ABIN967677

Détail du produit anti-BCL6 anticorps

Antigène: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCL6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. This product is sold under license to the following patent: US Patent No. 6,174,99
6. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Bcl-6 Recombinant Protein

Clone: K112-91

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968903, ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-BCL6 (ABIN967677)

Baumjohann, Okada, Ansel: "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2089-92, (2011) (PubMed).

Choi, Kageyama, Eto, Escobar, Johnston, Monticelli, Lao, Crotty: "ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6." dans: Immunity, Vol. 34, Issue 6, pp. 932-46, (2011) (PubMed).

Crotty: "Follicular helper CD4 T cells (TFH)." dans: Annual review of immunology, Vol. 29, pp. 621-63, (2011) (PubMed).

Crotty, Johnston, Schoenberger: "Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation." dans: Nature immunology, Vol. 11, Issue 2, pp. 114-20, (2010) (PubMed).

Fazilleau, McHeyzer-Williams, Rosen, McHeyzer-Williams: "The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding." dans: Nature immunology, Vol. 10, Issue 4, pp. 375-84, (2009) (PubMed).

Nurieva, Chung, Martinez, Yang, Tanaka, Matskevitch, Wang, Dong: "Bcl6 mediates the development of T follicular helper cells." dans: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1001-5, (2009) (PubMed).

Johnston, Poholek, DiToro, Yusuf, Eto, Barnett, Dent, Craft, Crotty: "Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation." dans: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1006-10, (2009) (PubMed).

Klein, Dalla-Favera: "Germinal centres: role in B-cell physiology and malignancy." dans: Nature reviews. Immunology, Vol. 8, Issue 1, pp. 22-33, (2007) (PubMed).

Ye, Lista, Lo Coco, Knowles, Offit, Chaganti, Dalla-Favera: "Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma." dans: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 747-50, (1993) (PubMed).

Détails sur BCL6

Antigène: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Autre désignation: BCL-6 (BCL6 Produits)

Synonymes: anticorps bcl5, anticorps laz3, anticorps bcl6a, anticorps znf51, anticorps zbtb27, anticorps MGC132260, anticorps Bcl5, anticorps BCL5, anticorps BCL6A, anticorps LAZ3, anticorps ZBTB27, anticorps ZNF51, anticorps B-cell CLL/lymphoma 6 L homeolog, anticorps B-cell CLL/lymphoma 6, anticorps B cell leukemia/lymphoma 6, anticorps bcl6.L, anticorps bcl6, anticorps BCL6, anticorps Bcl6

Sujet: The K112-91 monoclonal antibody specifically binds to Bcl-6. Bcl-6 was first identified as a proto-oncogene frequently deregulated by chromosomal translocations in non-Hodgkin B-cell lymphomas. It is a nuclear transcriptional repressor of the BTB/POZ zinc-finger family of transcription factors. In addition to its role in cancer, Bcl-6 plays an important role in normal lymphocyte differentiation. Bcl-6 is highly expressed in germinal center B cells, where it promotes the germinal center reaction by inducing proliferation and inhibiting the DNA-damage response. Additionally, Bcl-6 has been identified as a key factor in promoting the differentiation of CD4+ follicular T helper (Tfh) cells, which are involved in promoting germinal center formation and providing help to B cells. The interplay of Bcl-6 and another transcriptional repressor, Blimp-1, is thought to be critical in defining the results of both B-cell and T-cell differentiation.
Synonyms: BCL6, B-cell lymphoma 6 protein, LAZ3, Laz-3, ZBTB27, ZNF51

Poids moléculaire: 87-98 kDa

ID gène: 604, 12053

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus