HIF1AN Protein (AA 2-349) (His tag)

N° du produit ABIN3092929

Détail du produit

Antigène: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Type de proteíne: Recombinant

Attributs du protein: AA 2-349

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HIF1AN protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: AATAAEAVAS GSGEPREEAG ALGPAWDESQ LRSYSFPTRP IPRLSQSDPR AEELIENEEP VVLTDTNLVY PALKWDLEYL QENIGNGDFS VYSASTHKFL YYDEKKMANF QNFKPRSNRE EMKFHEFVEK LQDIQQRGGE ERLYLQQTLN DTVGRKIVMD FLGFNWNWIN KQQGKRGWGQ LTSNLLLIGM EGNVTPAHYD EQQNFFAQIK GYKRCILFPP DQFECLYPYP VHHPCDRQSQ VDFDNPDYER FPNFQNVVGY ETVVGPGDVL YIPMYWWHHI ESLLNGGITI TVNFWYKGAP TPKRIEYPLK AHQKVAIMRN IEKMLGEALG NPQEVGPLLN TMIKGRYN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HIF1AN Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Autre désignation: HIF1AN (HIF1AN Produits)

Synonymes: FIH1 Protein, 2310046M24Rik Protein, A830014H24Rik Protein, cb728 Protein, id:ibd5139 Protein, hypoxia inducible factor 1 alpha subunit inhibitor Protein, hypoxia-inducible factor 1, alpha subunit inhibitor Protein, hypoxia inducible factor 1, alpha subunit inhibitor Protein, hypoxia-inducible factor 1, alpha subunit inhibitor S homeolog Protein, HIF1AN Protein, Hif1an Protein, hif1an Protein, hif1an.S Protein

Sujet: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. {ECO:0000269|PubMed:12042299, ECO:0000269|PubMed:12080085, ECO:0000269|PubMed:17003112, ECO:0000269|PubMed:17573339, ECO:0000269|PubMed:18299578, ECO:0000269|PubMed:19245366, ECO:0000269|PubMed:21177872, ECO:0000269|PubMed:21251231}.

Poids moléculaire: 41.1 kDa Including tag.

UniProt: Q9NWT6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development