anti-Souris CD3E anticorps pour Cytotoxicity Test

Recommended CD3E Antibody (fourni par: Connectez-vous pour afficher )

Antigène
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Anticorps
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivité
Souris
507
482
121
87
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Hôte
Hamster arménien
460
231
200
63
21
15
3
1
1
Clonalité
Monoclonal
Conjugué
Cet anticorp CD3E est non-conjugé
96
79
76
37
25
22
21
20
20
18
17
17
17
14
14
14
14
12
12
12
12
10
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
739
338
243
203
150
144
111
97
96
42
30
18
13
9
8
7
6
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher
Request

Ce produit gratuit

Envoyez-nous votre proposition de validation. J'aimerais valider ce produit

Savoir plus

Images

N° du produit ABIN967568
79,99 €
Plus frais de livraison 40,00 € et TVA
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Connectez-vous pour afficher 145-2C11 13
1 ABIN1176869 BR CyTox FM IHC (fro) FACS IP WB Armenian Hamster IgG1, kappa Connectez-vous pour afficher 145-2C11 10

anti-CD3E anticorps pour Cytotoxicity Test mieux référencés

anti-CD3E Anticorps

Application / Reactivité Souris
Activity Assay (AcA) 1 Anticorps
Blocking Reagent (BR) 6 Anticorps
Cytometry by Time of Flight (CyTOF) 12 Anticorps
Cytotoxicity Test (CyTox) 3 Anticorps
Depletion (Dep) 3 Anticorps
ELISA 16 Anticorps
ELISA (Capture) 6 Anticorps
ELISpot (Capture) (ELISPOT (Capture)) 2 Anticorps
Flow Cytometry (FACS) 406 Anticorps
Fluorescence Microscopy (FM) 1 Anticorps
Functional Studies (Func) 12 Anticorps
Immunocytochemistry (ICC) 32 Anticorps
Immunofluorescence (IF) 56 Anticorps
Immunofluorescence (fixed cells) (IF/ICC) 7 Anticorps
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Anticorps
Immunohistochemistry (IHC) 105 Anticorps
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Anticorps
Immunohistochemistry (Frozen Sections) (IHC (fro)) 41 Anticorps

General

Antigène CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Anticorps
Reactivité Souris
(507), (482), (121), (87), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Hôte Hamster arménien
(460), (231), (200), (63), (21), (15), (3), (1), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp CD3E est non-conjugé
(96), (79), (76), (37), (25), (22), (21), (20), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(739), (338), (243), (203), (150), (144), (111), (97), (96), (42), (30), (18), (13), (9), (8), (7), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 13 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-CD3E anticorps

Détail du antigène CD3E Information d'application Stockage ProductDetails: References for anti-CD3E antibody (ABIN967568) Images
Marque BD Pharmingen™
Attributs du produit 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Clone 145-2C11
Isotype IgG1, kappa

Détail du antigène CD3E

Détail du produit anti-CD3E anticorps Information d'application Stockage ProductDetails: References for anti-CD3E antibody (ABIN967568) Images Haut de la page
Antigène
Autre désignation CD3e (CD3E Antibody Extrait)
Sujet The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CD3epsilon chain
Domaine de recherche CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Information d'application

Détail du produit anti-CD3E anticorps Détail du antigène CD3E Stockage ProductDetails: References for anti-CD3E antibody (ABIN967568) Images Haut de la page
Restrictions For Research Use only

Stockage

Détail du produit anti-CD3E anticorps Détail du antigène CD3E Information d'application ProductDetails: References for anti-CD3E antibody (ABIN967568) Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4° C.

ProductDetails: References for anti-CD3E antibody (ABIN967568)

Détail du produit anti-CD3E anticorps Détail du antigène CD3E Information d'application Stockage Images Haut de la page
Produit citée dans:

Martin, Lenardo: "Morphological, biochemical, and flow cytometric assays of apoptosis." dans: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 14, pp. Unit 14.13, 2008

Kruisbeek, Shevach, Thornton: "Proliferative assays for T cell function." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.12, 2008

Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." dans: International immunology, Vol. 9, Issue 7, pp. 935-44, 1997

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996

Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." dans: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, 1996

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." dans: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." dans: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Background publications

Isakov, Wange, Burgess, Watts, Aebersold, Samelson: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." dans: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995

Images

Détail du produit anti-CD3E anticorps Détail du antigène CD3E Information d'application Stockage ProductDetails: References for anti-CD3E antibody (ABIN967568) Haut de la page
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E anticorps (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained ...
 image for anti-CD3E anticorps (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) BALB/c thymocytes were also stained with purified anti-mouse CD3e 145-2C11 or isotype...
 image for anti-CD3E anticorps (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) Purified anti-mouse CD3e mAb 145-2C11