HMGN1 anticorps

N° du produit ABIN103313

N° du produit (Fournisseur): 200-301-278

L’anticorps Souris Monoclonal anti-HMGN1 a été validé pour WB, IHC et ELISA. Il convient pour détecter HMGN1 dans des échantillons de Humain.

Aperçu rapide pour HMGN1 anticorps (ABIN103313)

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: IPO-38

Détail du produit anti-HMGN1 anticorps

N° du produit (Fournisseur): 200-301-278

Fournisseur: Rockland

Fonction: IPO-38 Antibody

Réactivité croisée (Details): This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes.

Attributs du produit: Synonyms: mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody

Purification: Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Immunogen Type: Native Protein

Isotype: IgM kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation.  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsillar tissue is typically used as a positive control.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur HMGN1

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Autre désignation: Hmgn1

Sujet: Background: The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.

ID gène: 15312

NCBI Accession: NP_032277

UniProt: P18608

Pathways: Chromatin Binding