alpha 2 Macroglobulin anticorps (Biotin)

N° du produit ABIN103763

N° du produit (Fournisseur): 200-106-207-0100

Cet anticorps anti-alpha 2 Macroglobulin est un anticorps Chèvre Polyclonal détectant alpha 2 Macroglobulin dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour alpha 2 Macroglobulin anticorps (Biotin) (ABIN103763)

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha 2 Macroglobulin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-alpha 2 Macroglobulin anticorps (Biotin)

N° du produit (Fournisseur): 200-106-207-0100

Fournisseur: Rockland

Fonction: Alpha-2-Macroglobulin Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified alpha-2-MACROGLOBULIN [Human Plasma].

Attributs du produit: Synonyms: goat anti-Alpha-2-Macroglobulin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Purification: Anti-Alpha-2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: alpha-2-MACROGLOBULIN [Human Plasma]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Alpha-2-Macroglobulin has been tested by western blot and is suitable to be assayed against 1.0 μg of alpha-2-MACROGLOBULIN in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur alpha 2 Macroglobulin

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: A2M

Sujet: Background: Anti-alpha-2-Macroglobulin antibody is secreted into the plasma and belongs to the protease inhibitor I39 family. Anti-alpha-2-Macroglobulin inhibits all proteinases utilizing a ""bait region,"" or specific cleavage site, which traps the proteinase, after which a thioester bond is hydrolyzed and regulates the covalent binding of the protein to proteinase. Anti-alpha-2-Macroglobulin antibody is ideal for investigators interested in Cardiovascular , Protease Inhibitor, or Cell Biology research.

ID gène: 2

NCBI Accession: NP_000005

UniProt: P01023

Pathways: Lipid Metabolism