CD4 anticorps

N° du produit ABIN1042376

Il existe 3+ publications pour ce produit. L’anticorps anti-CD4 Monoclonal Souris est utilisé pour la détection de CD4 dans des échantillons de Humain. Il a été validé pour FACS, IHC, IF, Func, IHC (p), StM, Q, Diff et D.

Aperçu rapide pour CD4 anticorps (ABIN1042376)

Antigène: CD4

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Staining Methods (StM), Quantification (Q), Differentiation (Diff), Detection (D)

Clone: Edu-2

Détail du produit anti-CD4 anticorps

Fonction: Mouse Anti Human CD4

Specificité: CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).

Attributs du produit: CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.

Immunogène: Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 μL/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 μL of the purified monoclonal antibody with RaM FITC conjugate and 100 μL blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 1 mL of 100 μg/mL purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2 % BSA, 0.09 % sodium azide, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Références pour anticorps anti-CD4 (ABIN1042376)

Allison, Lanier: "Structure, function, and serology of the T-cell antigen receptor complex." dans: Annual review of immunology, Vol. 5, pp. 503-40, (1987) (PubMed).

Kaneoka, Perez-Rojas, Sasasuki, Benike, Engleman: "Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 158-64, (1983) (PubMed).

Friedrich, OReilly, Koziner, Gebhard, Good, Evans: "T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions." dans: Blood, Vol. 59, Issue 4, pp. 696-701, (1982) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events