Interferon gamma anticorps

N° du produit ABIN1043777

N° du produit (Fournisseur): 201-401-c41

L’anticorps Lapin Polyclonal anti-Interferon gamma a été validé pour WB et ELISA. Il convient pour détecter Interferon gamma dans des échantillons de Boeuf (Vache). Il y a 2+ publications disponibles.

Aperçu rapide pour Interferon gamma anticorps (ABIN1043777)

Antigène: Interferon gamma (IFNG)

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Interferon gamma est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Interferon gamma anticorps

N° du produit (Fournisseur): 201-401-c41

Fournisseur: Rockland

Fonction: Interferon Gamma Antibody

Réactivité croisée (Details): This antibody is specific for bovine IFN gamma protein.

Attributs du produit: Synonyms: rabbit anti-IFN gamma, rabbit anti-Interferon gamma, IFNG, BoIFNG

Purification: This product was protein A purified from monospecific antiserum by chromatography.

Immunogène:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.59 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Interferon gamma (ABIN1043777)

George, Ma, Motyka, Shi, Liu, Griebel: "A dendritic cell-targeted chimeric hepatitis B virus immunotherapeutic vaccine induces both cellular and humoral immune responses in vivo." dans: Human vaccines & immunotherapeutics, Vol. 16, Issue 4, pp. 779-792, (2020) (PubMed).

Schroder, Hertzog, Ravasi, Hume: "Interferon-gamma: an overview of signals, mechanisms and functions." dans: Journal of leukocyte biology, Vol. 75, Issue 2, pp. 163-89, (2004) (PubMed).

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFN gamma

Sujet: Background: Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

ID gène: 281237

NCBI Accession: NP_776511

UniProt: A9QXB7

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy