IL-3 anticorps (Biotin)

N° du produit ABIN1043808

N° du produit (Fournisseur): 209-406-b92

Cet anticorps anti-IL-3 est un anticorps Lapin Polyclonal détectant IL-3 dans WB, ELISA et IHC. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour IL-3 anticorps (Biotin) (ABIN1043808)

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-3 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-IL-3 anticorps (Biotin)

N° du produit (Fournisseur): 209-406-b92

Fournisseur: Rockland

Fonction: IL-3 Antibody Biotin Conjugated

Attributs du produit: Synonyms: rabbit anti-IL-3 biotin conjugated antibody, rabbit anti-Interleukin 3 biotin conjugated antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

Purification: Biotin Conjugated Anti-Human IL-3 Antibody Biotin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogène:

Immunogen: Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IL-3 (Biotin) (ABIN1043808)

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." dans: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, (2010) (PubMed).

Détails sur IL-3

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL3

Sujet: Background: The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

ID gène: 3562

NCBI Accession: NP_000579

UniProt: P08700

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy