CXCR4 anticorps (C-Term)

N° du produit ABIN1048478

L’anticorps Lapin Polyclonal anti-CXCR4 a été validé pour IHC et IHC (p). Il convient pour détecter CXCR4 dans des échantillons de Humain, Souris, Rat, Singe, Lapin, Porc, Boeuf (Vache), Chien, Cheval, Hamster et Roussette (Chauve-souris).

Aperçu rapide pour CXCR4 anticorps (C-Term) (ABIN1048478)

Antigène: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivité: Humain, Souris, Rat, Singe, Lapin, Porc, Boeuf (Vache), Chien, Cheval, Hamster, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCR4 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CXCR4 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human CXCR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Mouse, Rat, Bat, Bovine, Dog, Cat, Hamster, Panda, Rabbit, Horse, Pig, Opossum, Lizard (100%) Elephant, Turkey, Chicken (94%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 17 amino acid peptide from C-terminus of human CXCR4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Mouse, Rat, Bat, Bovine, Dog, Cat, Hamster, Panda, Rabbit, Horse, Pig, Opossum, Lizard (100%), Elephant, Turkey, Chicken (94%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (1.6 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CXCR4

Antigène: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Autre désignation: CXCR4

Sujet: Name/Gene ID: CXCR4
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR4, CD184, CD184 antigen, Cmkar4, Cxc chemokine receptor 4, CXCR-4, CXC-R4, D2S201E, FB22, Fusin, HM89, LCR1, NPY3R, NPYRL, NPYY3R, Npyy3, HSY3RR, SDF-1 receptor, WHIM, C-x-c chemokine receptor 4, LESTR, Neuropeptide Y receptor Y3, NPYR

ID gène: 7852

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events