EPH Receptor B2 anticorps (Internal Region)

N° du produit ABIN1048573

Cet anticorps Lapin Polyclonal détecte spécifiquement EPH Receptor B2 dans IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Poisson zèbre (Danio rerio), Singe et Lapin.

Aperçu rapide pour EPH Receptor B2 anticorps (Internal Region) (ABIN1048573)

Antigène: EPH Receptor B2 (EPHB2)

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Singe, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EPH Receptor B2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EPH Receptor B2 anticorps

Épitope: Internal Region

Marque: IHC-plus™

Specificité: Human EPHB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%) Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%) Xenopus, Seabass (87%) Stickleback (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 15 amino acid peptide from internal region of human EPHB2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%), Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%), Xenopus, Seabass (87%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (7 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur EPH Receptor B2

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: EPHB2 / EPH Receptor B2

Sujet: Name/Gene ID: EPHB2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHB2, CAPB, Cek5, EK5, ETECK, EPH tyrosine kinase 3, DRT, ELK-related tyrosine kinase, Hek5, EPH-like kinase 5, EPHT3, Nuk, PCBC, Tyrosine-protein kinase TYRO5, Protein-tyrosine kinase HEK5, Tyro5, EPH receptor B2, Ephrin type-B receptor 2, EPTH3

ID gène: 2048

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins