EPH Receptor B2 anticorps (Internal Region)
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- Antigène Voir toutes EPH Receptor B2 (EPHB2) Anticorps
- EPH Receptor B2 (EPHB2)
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Épitope
- Internal Region
- Reactivité
- Humain, Souris, Rat, Singe, Lapin, Poisson zèbre (Danio rerio)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp EPH Receptor B2 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human EPHB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%) Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%) Xenopus, Seabass (87%) Stickleback (80%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 15 amino acid peptide from internal region of human EPHB2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%), Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%), Xenopus, Seabass (87%), Stickleback (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EPHB2 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (7 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- EPH Receptor B2 (EPHB2)
- Autre désignation
- EPHB2 / EPH Receptor B2 (EPHB2 Produits)
- Synonymes
- anticorps CAPB, anticorps DRT, anticorps EK5, anticorps EPHT3, anticorps ERK, anticorps Hek5, anticorps PCBC, anticorps Tyro5, anticorps ERK2, anticorps ERT1, anticorps MAPK2, anticorps P42MAPK, anticorps PRKM1, anticorps PRKM2, anticorps p38, anticorps p40, anticorps p41, anticorps p41mapk, anticorps RGD1564232, anticorps Cek5, anticorps Drt, anticorps ETECK, anticorps Erk, anticorps Nuk, anticorps Prkm5, anticorps Qek5, anticorps Sek3, anticorps cek5, anticorps ephb2, anticorps xEphB2, anticorps si:ch73-160e9.1, anticorps tpx2, anticorps wu:fb09e10, anticorps wu:fc64a03, anticorps wu:fi18g12, anticorps zgc:112296, anticorps EPH receptor B2, anticorps mitogen-activated protein kinase 1, anticorps Eph receptor B2, anticorps EPH receptor B2 L homeolog, anticorps eph receptor B2a, anticorps eph receptor B2b, anticorps EPHB2, anticorps MAPK1, anticorps Ephb2, anticorps ephb2.L, anticorps ephb2a, anticorps ephb2b
- Sujet
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Name/Gene ID: EPHB2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHB2, CAPB, Cek5, EK5, ETECK, EPH tyrosine kinase 3, DRT, ELK-related tyrosine kinase, Hek5, EPH-like kinase 5, EPHT3, Nuk, PCBC, Tyrosine-protein kinase TYRO5, Protein-tyrosine kinase HEK5, Tyro5, EPH receptor B2, Ephrin type-B receptor 2, EPTH3 - ID gène
- 2048
- Pathways
- Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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