FZD8 anticorps (N-Term)

N° du produit ABIN1048631

Détail du produit anti-FZD8 anticorps

Antigène: FZD8 (Frizzled Family Receptor 8 (FZD8))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Xenopus laevis, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD8 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human FZD8 / Frizzled 8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Horse, Opossum, Xenopus (100%) Lizard, Seabass, Pufferfish (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 12 amino acid peptide from N-terminal extracellular domain of human FZD8 / Frizzled 8. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Horse, Opossum, Xenopus (100%), Lizard, Seabass, Pufferfish (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (7 - 17 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FZD8

Antigène: FZD8 (Frizzled Family Receptor 8 (FZD8))

Autre désignation: FZD8 / Frizzled 8 (FZD8 Produits)

Synonymes: anticorps FZ-8, anticorps hFZ8, anticorps FZD8, anticorps Fz-8, anticorps frizzled8, anticorps xfz8, anticorps Xfz8, anticorps frizzled-8, anticorps hfz8, anticorps Fz8, anticorps fzd8, anticorps fz5, anticorps fz8a, anticorps fzc, anticorps wu:fd02c05, anticorps zg05, anticorps ZG06, anticorps Zfz8b, anticorps fz6, anticorps fz8b, anticorps fzA, anticorps ik:tdsubc_1e1, anticorps xx:tdsubc_1e1, anticorps zfzA, anticorps frizzled class receptor 8, anticorps frizzled class receptor 8 S homeolog, anticorps frizzled class receptor 8 L homeolog, anticorps frizzled class receptor 8a, anticorps frizzled class receptor 8b, anticorps FZD8, anticorps fzd8.S, anticorps fzd8, anticorps Fzd8, anticorps fzd8.L, anticorps fzd8a, anticorps fzd8b

Sujet: Name/Gene ID: FZD8
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD8, Frizzled family receptor 8, Frizzled homolog 8, FZ-8, Fz8, Frizzled 8, Frizzled-8, HFZ8

ID gène: 8325

Pathways: Signalisation WNT