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GPER anticorps (Extracellular Domain)

GPER Reactivité: Humain, Singe WB, IHC (p), IHC, IF Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048794
  • Antigène Voir toutes GPER Anticorps
    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))
    Épitope
    • 16
    • 16
    • 16
    • 14
    • 9
    • 9
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 105
    • 63
    • 55
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    Humain, Singe
    Hôte
    • 105
    • 1
    Lapin
    Clonalité
    • 106
    Polyclonal
    Conjugué
    • 38
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Cet anticorp GPER est non-conjugé
    Application
    • 80
    • 45
    • 39
    • 39
    • 25
    • 20
    • 12
    • 9
    • 7
    • 5
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Marque
    IHC-plus™
    Specificité
    Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Dog, Opossum (94%) Panda, Bat, Horse, Rabbit, Platypus (88%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Dog, Opossum (94%), Panda, Bat, Horse, Rabbit, Platypus (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPER Anticorps primaire
  • Indications d'application
    Approved: IF, IHC, IHC-P (6.4 μg/mL), WB

    Usage: Antibody does not work for Western blot with mouse samples.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))
    Autre désignation
    GPER1 / GPR30 (GPER Produits)
    Synonymes
    anticorps CEPR, anticorps CMKRL2, anticorps DRY12, anticorps FEG-1, anticorps GPCR-Br, anticorps GPER, anticorps GPR30, anticorps LERGU, anticorps LERGU2, anticorps LyGPR, anticorps 6330420K13Rik, anticorps Ceprl, anticorps Gper, anticorps Gpr30, anticorps GPR41, anticorps GPCR30, anticorps G protein-coupled estrogen receptor 1, anticorps GPER1, anticorps GPER, anticorps Gper1
    Sujet
    Name/Gene ID: GPER1
    Subfamily: Transmembrane Estrogen Receptor
    Family: GPCR

    Synonyms: GPER1, CMKRL2, CEPR, FEG-1, G-protein coupled receptor 30, G protein-coupled receptor 30, GPCR-Br, GPER, Heptahelix receptor, LERGU, LyGPR, MER, GPR30, IL8-related receptor DRY12, LERGU2, Chemokine receptor-like 2, DRY12, Membrane estrogen receptor
    ID gène
    2852
    Pathways
    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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