PCNA anticorps (AA 111-122)

N° du produit ABIN1049498

L’anticorps Chèvre Polyclonal anti-PCNA a été validé pour WB, IHC et ELISA. Il convient pour détecter PCNA dans des échantillons de Humain, Souris, Rat et Porc.

Aperçu rapide pour PCNA anticorps (AA 111-122) (ABIN1049498)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-PCNA anticorps

Épitope: AA 111-122

Fonction: PCNA (aa111-122)

Séquence: EAPNQEKVSD YE

Specificité: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-EAPNQEKVSDYE, from the internal region of the protein sequence according to NP_002583.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8 kDa according to Human NP_002583.1). Approx.35 kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33 kDa in Pig Spleen

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

ID gène: 5111, 18538, 25737

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy