C3 anticorps (AA 681-693)

N° du produit ABIN1049513

L’anticorps Chèvre Polyclonal anti-C3 a été validé pour ELISA. Il convient pour détecter C3 dans des échantillons de Humain.

Aperçu rapide pour C3 anticorps (AA 681-693) (ABIN1049513)

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp C3 est non-conjugé

Application: ELISA

Classe de qualité: Recent

Détail du produit anti-C3 anticorps

Épitope: AA 681-693

Fonction: complement C3 (aa681-693)

Séquence: DKVGKYPKEL RKC

Specificité: This antibody is expected to recognize the C3 alpha chain and C3a anaphylatoxin,

Homologie: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence DKVGKYPKELRKC, from the internal region of the protein sequence according to NP_000055.2.

Isotype: IgG

Information d'application

Indications d'application:

DS WB Results: Preliminary experiments gave an approx 125 kDa band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field. Please note that a different antibody developed to a different part of the protein showed identical results: EB11703.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur C3

Antigène: C3 (Complement Component 3 (C3))

Autre désignation: Complement Component 3

Sujet: C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3

ID gène: 718

NCBI Accession: NP_000055

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling