CCL5 anticorps (AA 24-91)

N° du produit ABIN1078469

Détail du produit anti-CCL5 anticorps

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Épitope: AA 24-91

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGHHHHHHSG SEF-SPYASDT TPCCFAYISR PLPRTHVQEY FYTSSKCSMA AVVFITRKNR QVCANPEKKW VREYINALEL S

Specificité: It has been selected for its ability to recognize RANTES in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant RANTES expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against RANTES.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant RANTES (Ser24~Ser91) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur CCL5

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) (CCL5 Produits)

Synonymes: anticorps Rantes, anticorps Scya5, anticorps D17S136E, anticorps RANTES, anticorps SCYA5, anticorps SIS-delta, anticorps SISd, anticorps TCP228, anticorps eoCP, anticorps MuRantes, anticorps C-C motif chemokine 5-like, anticorps C-C motif chemokine ligand 5, anticorps chemokine (C-C motif) ligand 5, anticorps LOC100230935, anticorps CCL5, anticorps Ccl5

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration