GM-CSF anticorps
Aperçu rapide pour GM-CSF anticorps (ABIN1100979)
Antigène
Voir toutes GM-CSF (CSF2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This purified antibody has been heated to 56 °C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
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Purification
- Purified, heated to 56°C for 30 minutes
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Immunogène
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
Type of Immunogen: Recombinant protein -
Isotype
- IgG
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Indications d'application
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Approved: ELISA, WB (1:500 - 1:2000)
Usage: This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.5 kD in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- deionized water
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Concentration
- Lot specific
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Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
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Conseil sur la manipulation
- avoid freeze thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Autre désignation
- CSF2 / GMCSF / GM-CSF
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Sujet
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Name/Gene ID: CSF2
Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF -
ID gène
- 1437
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UniProt
- P04141
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Pathways
- Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin
Antigène
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