IGF2 anticorps
Aperçu rapide pour IGF2 anticorps (ABIN1102257)
Antigène
Voir toutes IGF2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Marque
- IHC-plus™
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Specificité
- Human IGF-II
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human IGF-II.
Type of Immunogen: Cell extract -
Isotype
- IgG
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Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (3 - 5 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1-4.6 μg/mL of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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Autre désignation
- IGF2
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Sujet
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Name/Gene ID: IGF2
Family: Hormone
Synonyms: IGF2, C11orf43, Insulin-like growth factor II, IGF-II, PP9974, Somatomedin-A -
ID gène
- 3481
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UniProt
- P01344
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Pathways
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
Antigène
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