TRAIL anticorps

N° du produit ABIN1104738

Cet anticorps anti-TRAIL Polyclonal Lapin (ABIN1104738) détecte spécifiquement TRAIL dans WB, IHC, ELISA, IHC (p) et Neut. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TRAIL anticorps (ABIN1104738)

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRAIL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Détail du produit anti-TRAIL anticorps

Specificité: Human TRAIL/Apo2L

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human TRAIL/Apo2L.

Type of Immunogen: Cell extract

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7420 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/mL), a concentration of 150 ng/mL of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur TRAIL

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TNFSF10 / TRAIL

Sujet: Name/Gene ID: TNFSF10
Family: TNF

Synonyms: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253 antigen, TRAIL, Protein TRAIL, TL2

ID gène: 8743

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity