CD14 anticorps

N° du produit ABIN1105870

Cet anticorps Rat Monoclonal détecte spécifiquement CD14 dans WB, IF et Func. Il présente une réactivité envers Souris.

Aperçu rapide pour CD14 anticorps (ABIN1105870)

Antigène: CD14

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func)

Clone: 14-02-14

Détail du produit anti-CD14 anticorps

Specificité: The monoclonal antibody Sa14-2 recognizes the mouse monocyte marker CD14.

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography

Immunogène: CD14 transfectant

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8 °C.

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: The CD14 receptor is a pattern recognition molecule in the innate immune response against microorganisms and other exogenous and endogenous stress factors. CD14 was characterized as a receptor for LPS. The CD14 gene consists of two exons which code for a single mRNA that is translated into a protein of 375 amino acids. The CD14 protein is composed of eleven leucin-rich repeats, which are also found in TLR and which are important in PAMP binding. In contrast to TLR, however, CD14 lacks a transmembrane domain, and thus cannot initiate intracellular signal transduction by itself. The most important CD14 signaling co-receptor is toll-like receptor 4 (TLR4), which activates, among others, the nuclear factor κB (NF-κB) inflammatory pathway. The CD14 protein is processed in the endoplasmatic reticulum and expressed as a 55 kDa glycoprotein on the cell surface via a glycosylphosphatidyl (GPI) anchor. Like other GPI-anchored proteins, CD14 accumulates on the cell surface in microdomains known as lipid rafts. CD14 is expressed pre dominantly on the surface of 'myeloid' cells, such as monocytes, macrophages and neutrophils, but at lower levels also on epithelial cells, endothelial cells and fibroblasts. CD14 is also expressed in a soluble form (sCD14). sCD14 may result from secretion of the protein before coupling to the GPI anchor or from shedding or cleavage from the surface of monocytes. sCD14 is present in the circulation and other body fluids and levels of sCD14 in plasma increase during inflammation and infection. CD14 is a molecule with a broad range of functions. In addition to functioning as a pattern recognition receptor for a variety of microbial ligands, CD14 also acts as a receptor for endogenous molecules like intercellular adhesion molecule (ICAM)-3 on the surface of apoptotic cells, amyloid peptid, ceramide, and urate crystals. Ligation of CD14 by these ligands, except for apoptotic cells, mediates activation of inflammatory responses.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

ID gène: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades