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CD14 anticorps (AA 20-345)

Name: CD14 anticorps (AA 20-345)
Brand: antibodies-online
SKU: ABIN7160185
Price: 415 EUR
Availability: InStock

CD14 Reactivité: Humain FACS, WB, IHC, ELISA, IF Hôte: Souris Monoclonal 14G1F3 unconjugated

Western Blot Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue All lanes: CD14 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 anticorps (AA 20-345))

Immunofluorescence staining of A549 cells with ABIN7160185 at 1:90, counter-stained with DAPI. (CD14 anticorps (AA 20-345))

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate All lanes: CD14 antibody at 1:1800 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 anticorps (AA 20-345))

IHC image of ABIN7160185 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD14 anticorps (AA 20-345))

9 images

N° du produit ABIN7160185

Fiche de données

Antigène Voir toutes CD14 Anticorps

CD14
Épitope
16

9

7

5

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-345
Reactivité
268

55

32

30

29

28

25

24

24

18

17

14

10

9

8

8

8

5

2

2

2

1

1

1
Humain
Hôte
224

64

25

3

1
Souris
Anticorps secondaires appropriés
Clonalité
253

64
Monoclonal
Conjugué
128

30

24

18

15

8

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CD14 est non-conjugé
Application
223

103

63

59

53

49

22

22

21

14

14

13

7

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Réactivité croisée

Humain, Souris, Lapin

Purification

Protein A purified

Immunogène

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Clone

14G1F3

Isotype

IgG1, IgG1 kappa

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anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivité: Humain, Souris, Rat, Chien, Mouton, Lapin WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Indications d'application

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

CD14

Autre désignation

CD14 (CD14 Produits)

Synonymes

anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14

Sujet

Background: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
Aliases: Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form], CD14

UniProt

P08571

Pathways

Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades