FAK anticorps (AA 706-1052)

N° du produit ABIN1107179

L’anticorps anti-FAK Monoclonal Souris est utilisé pour la détection de FAK dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF et IP.

Aperçu rapide pour FAK anticorps (AA 706-1052) (ABIN1107179)

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 12G4

Détail du produit anti-FAK anticorps

Épitope: AA 706-1052

Specificité: This antibody detects human, mouse and rat FAK in Western blotting, Immunoprecipitation and immunocytostaining. This antibody detects ~125 kDa of human FAK on Westernblotting with whole cell lysates from human cell line such as HeLa. Since active FAK is highly phosphorylated, position of FAK band on westernblotting may varry with its phosphorylation level.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein-A Sepharose

Immunogène: GST fusion protein containing human FAK (residues 706-1052 a.a.)

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 50 % glycerol, without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store (in aliquots) at -20 °C.

Détails sur FAK

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: FAK1 / PTK2

Sujet: FAK (focal adhesion kinase) is a protein tyrosine kinase which colocalizes with integrin receptors at cell-substratum contact sites (focal adhesions). It is thought that enhanced tyrosine phosphorylation of FAK is important for integrin mediated signaling as well as for other signalings. FAK associates with a number of proteins such as Src, Shc, PI-3 kinase, Grb2, Cas, Cas-L, paxilin and transmamblane proteins, which enables FAK to couple to a variety of different intracellular transduction pathways in adherent and non-adherent cells. FAK is involved in anchorage-dependent survival signal through PI-3 kinase and also may play a role in cell growth, migration, cytoskeletal organization.Synonyms: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK

ID gène: 5747

NCBI Accession: NP_722560

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling