ENO2/NSE anticorps

N° du produit ABIN110966

L’anticorps anti-ENO2/NSE Monoclonal Souris est utilisé pour la détection de ENO2/NSE dans des échantillons de Humain. Il a été validé pour WB, IHC (p), IHC (fro) et EIA.

Aperçu rapide pour ENO2/NSE anticorps (ABIN110966)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 5G10

Détail du produit anti-ENO2/NSE anticorps

Specificité: This antibody is specific for Human Neuron Specific Enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma. Reacts with a 45 kDa band corresponding to NSE on Immunoblots of brain extracts.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: Native NSE protein from Human brain.

Isotype: IgG2b

Information d'application

Indications d'application: ELISA. Immunohistology on Paraffin Sections: 1/100-1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Control: Brain cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: Neuron Specific Enolase

Sujet: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase

ID gène: 2026

NCBI Accession: NP_001966

UniProt: P09104