C3 anticorps

N° du produit ABIN111064

Cet anticorps Souris Monoclonal détecte spécifiquement C3 dans WB, IHC (fro) et EIA. Il présente une réactivité envers Humain, Cobaye et Lapin.

Aperçu rapide pour C3 anticorps (ABIN111064)

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Humain, Cobaye, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: H13

Détail du produit anti-C3 anticorps

Specificité: This Monoclonal antibody Clone H 13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H 13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with H 13.

Réactivité croisée (Details): Species reactivity (tested):Human, Guinea Pig and Rabbit.

Purification: Protein A Chromatography

Immunogène: Human complement component C3

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on frozen sections: 1: 10. Western Blot. ELISA. Detection of C3 deposits in tissue, cells, micro-organisms and immune complexes. Itshould be used with positive control anti-C3b (H11/H206). Non-activated, absorbed C3contains the C3a determinant, whereas activated C3 is negative with H 13
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur C3

Antigène: C3 (Complement Component 3 (C3))

Autre désignation: Complement C3

Sujet: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

ID gène: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling